Effects of TNF-? on changes of inducible nitric oxide synthase-nitric oxide system activity induced by arginine vasopressin in rat cardiac fibroblasts
- VernacularTitle:TNF-?对精氨酸加压素诱导下大鼠心肌成纤维细胞诱导型一氧化氮合酶-一氧化氮系统活性的影响
- Author:
Yanhong FAN
;
Lianyou ZHAO
;
Haichang WANG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor;
Argipressin;
Cardiac fibroblasts;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of tumor necrosis factor-? (TNF-?) on inducible nitric oxide synthase (iNOS)-nitric oxide (NO) system activity in arginine vasopressin (AVP)-induced rat cardiac fibroblasts (CFs). METHODS: CFs were isolated by trypsin digestion method. Nitrate reductase method, spectrophotometry and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect NO contents, NOS activity and iNOS mRNA expression, respectively. RESULTS: AVP significantly increased iNOS mRNA expression, NOS activity and NO contents in CFs. Combined with AVP, TNF-? enhanced the effects of AVP on iNOS-NO system activity in a concentration-dependent manner. However, if the concentration of TNF-? was too high, the iNOS-NO system activity did not increase accordingly, but slightly decreased instead. CONCLUSION: TNF-? stimulates iNOS-NO system activity in coordination with AVP in CFs. The enhancement of NO contents inhibits ventricular remodeling induced by AVP and TNF-?. [
