Effects of dexamethasone on the changes of AP-1 and CREB activities in lung tissue after intravenous lipolysaccharide in rats
- VernacularTitle:活化蛋白-1和cAMP反应元件结合蛋白在脂多糖注射大鼠肺组织中的变化及地塞米松的影响
- Author:
Xin HONG
;
Zhenhui GUO
;
Baoling MAO
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
Activated protein-1;
cAMP response element binding protein;
Lipopolysaccharides;
Rats;
Respiratory distress syndrome, adult
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to explore the role of activator protein-1 (AP-1) and cAMP response element binding protein (CREB) in acute lung injury and anti-inflammatory mechanism of glucocorticoid. METHODS: Using the acute lung injury (ALI) model of rats by intravenous lipopolysaccharide (LPS, 5 mg/kg), the activities of AP-1 and CREB by electrophoretic mobility shift assay (EMSA) in lung tissue and effects of dexamethasone on the activities were observed. RESULTS: After injection of LPS, the peak activity of AP-1 was observed at 2 hours and it returned to normal in 12 hours. CREB peak activity was at 1 hours but it did not return to normal in 12 hours. Dexamethasone significantly inhibited the activities of AP-1 and CREB. CONCLUSIONS: AP-1 and CREB may play important roles in ALI in rats. One of the important anti-inflammatory mechanisms of glucocorticoid is its transcription al modification. [
