Overexpression and constitutive activation of MARCKS in brain of rats with multi-cerebral infarction
- VernacularTitle:急性多发脑梗塞大鼠海马皮层MARCKS蛋白的表达与活性的变化
- Author:
Wen BAI
;
Yunling ZHANG
;
Zhenyun HAN
;
Hong ZHENG
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Multi-cerebral infarction;
MARCKS;
Phosphorylation
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The purpose of this study was to investigate the expression of myristoylated alanine-rich C kinase substrate (MARCKS) and p-MARCKS during cerebral ischemia. METHODS: The multi-cerebral infarction model was established by method of Kaneko. The stroke symptoms and signs scoring system were used to evaluate the animal model. Microscope and electronic microscope were used to watch the structure of rat brain. The expression of MARCKS and p-MARCKS was measured by Western blotting. The expression pattern of these proteins was further analyzed for their distribution at cellular level by immunohistochemistry staining of the tissues. RESULTS AND CONCLUSION: The expression of MARCKS and p-MARCKS protein in acute ischemic brain were elevated compared with the normal and control group (P
