Effects of ?-amyloid protein on cultured neural stem cells in vitro
- VernacularTitle:?-淀粉样蛋白对体外培养的神经干细胞作用研究
- Author:
Xianmin XU
;
Bo NIU
;
Tingjie WANG
;
Qi YANG
;
Yuehong ZHANG
;
Dayong CAI
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Stem cells;
Amyloid beta-protein
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of ?-amyloid protein (A?) on neural stem cells cultured in vitro. METHODS: Neural stem cells (NSC) were isolated from E13 SD rats and cultured in serum-free medium (DMEM/F12). After detected by nestin, the A? was added to the NSC medium to observe the viability and proliferation of NSC by MTT, cell count and flow-cytometric examination. The effects of A? on differentiated NSC were also observed. RESULTS: A? markedly inhibited the proliferation and the cell viability of NSC when its concentration was higher than 25 ?mol/L. The differentiatory ability of NSC was inhibited when A? was in very low concentration. CONCLUSION: A? significantly inhibits the proliferation and differentiation of NSC and this may be one of the reasons that Alzheimer's disease is induced. [
