Regulatory mechanism in expression of IRS-1 and 2 by estrogen and high concentration of insulin
- VernacularTitle:雌激素和高胰岛素调节胰岛素受体底物-1,2表达机制研究
- Author:
Ping XIE
;
Meilian LIU
;
Weimin ZENG
;
Jianjun HUANG
;
Shuhua CHEN
;
Jin LU
;
Xia XU
;
Huiping SONG
- Publication Type:Journal Article
- Keywords:
Estrogens;
Insulin;
Receptor, insulin
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the molecular mechanism in modulation of expression of insulin receptor substrate-1 and-2 (IRS-1,-2) by estrogen and high concentration of insulin. METHODS: The 5′-regulatory regions of IRS-1 and IRS-2 gene were cloned into the pGL3 plasmid with luciferase reporter, and the clones were transfected into HeLa cells. The cells were incubated with estradiol (1 nmol/L) and high concentration of insulin (100 nmol/L). The relatively transcriptional activity of the 5′-regulatory regions of IRS-1 and IRS-2 gene was detected. RESULTS: It was found that the relatively transcriptional activity of the 5′-modulatory regions of IRS-2 reduced markedly after cells were incubated with 100 nmol/L insulin (P