Role of connective tissue growth factor in the transdifferentiation of renal epithelial cells induced by transforming growth factor-?
- VernacularTitle:结缔组织生长因子在转化生长因子?诱导的肾小管上皮细胞转分化中的作用
- Author:
Haiyan ZHANG
;
Youji LI
;
Yong DU
;
Xiaoyan LI
;
Xueqing YU
;
Rengao YE
- Publication Type:Journal Article
- Keywords:
Connective tissue;
Growth substances;
Transforming growth factor beta;
Epithelial cells
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of connective tissue growth factor (cTGF) in transforming growth factor-?-induced tubular-epithelial myofibroblasts trnansdifferentiation. METHODS: NRK52E cells were used and divided into several groups. The morphological changes were observed by light and electron microscopy (scanning and transmission). The expressions of cytokeratin-18 and ?-SMA were measured by immunohistochemistry. RT-PCR and Western-blot were used to detect the expression of collagen type I. RESULTS: Cultured with TGF-?_1 at concentration of 10 ?g/L for 3 days, part of the NRK52E cells developed elongated shape, loss of microvilli and apical-basal polarity, and appeared bundles of actin microfilaments. The addition of 10 mg/L TGF-?_1 neutralizing-antibody and 30 mg/L CTGF antisense oligonucleotides (ASON) almost completely blocked the morphological changes induced by TGF-?_1, only a little hypertrophy was observed. Cultured with 10 ?g/L TGF-?_1 for 3 days, the expression of cytokeratin-18 significantly decreased, and ?-SMA and collagen type I significantly increased. Treated with neutralizing-antibody, the expressions of cytokeratin-18, ?-SMA and collagen type I were almost restored. Changes induced by TGF-?_1 were not reversed by CTGF sense oligonucleotides (SON). CONCLUSION: It is demonstrated that CTGF as a TGF-? downstream cytokine mediates tubular-epithelial myofibroblast transdifferentiation. [
