Determination of the Content of Hesperidin in Weisu Granules by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定胃舒颗粒中橙皮苷的含量
- Author:
Yan QIAN
;
Chunjing ZHAO
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Weisu granules;
Hesperidin;
Content determination
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for determination of the content of hesperidin in weisu gran?ules.METHODS:Symmetry C 18 (150mm?3.9mm,5?m)column;acetonitrile-water(19.5∶80.5),water containing phosphoric acid(800∶0.1)as mobile phase and detection wavelength was284nm.RESULTS:The calibration curve was linear in sample size range of hesperidin of0.0228?g～0.1596?g(r=0.9996).The average recovery was97.2%(RSD=1.1%,n=6).CONCLU_ SION:The method was simple and accurate,and can be used for quality control of weisu granules.
