IGF-Ⅱ Promotes the Proliferation and Apoptosis of Human Colorectal Adenocarcinomas and Its Clinical Significance
- VernacularTitle:IGF-Ⅱ促进结直肠腺癌细胞增殖和凋亡及其临床意义
- Author:
Mingsheng ZHANG
;
Yunfeng ZHOU
;
Aihua HU
- Publication Type:Journal Article
- Keywords:
IGF-Ⅱ;
Colorectal Adenocarcinoma;
In situ hybridization
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship among IGF-II expression and cell proliferation and apoptosis in human colorectal adenocarcinoma. Methods 60 paraffin embedded samples of human colorectal adenocarcinomas were used in this study. IGF-ⅡmRNA expression was detected by in situ hybridization, proliferation cell nuclear antigen(PCNA) protein was measured by immunohistochemical staining and apoptosis was determined by TUNEL technology. Fifteen normal colorectal tissues were used as controls. Computerized image analysis system qualitatively analyzed the positive ratio of above three indices in the cancer and normal tissues. Results The percentage of IGF-ⅡmRNA expression was significantly higher in colorectal adenocarcinoma (39.64%?7.32%) than that in normal colorectal tissues (23.21%?4.10%) (P
