The Effects of ?-aescin on Germ Cell Apoptosis in the Bilateral Testes Following Ipsilateral Testicular Torsion and Detorsion
- VernacularTitle:?-七叶皂甙钠对睾丸扭转/复位后生精细胞凋亡的影响
- Author:
Xiqing GUI
;
Jianping HUANG
;
Conghui HAN
- Publication Type:Journal Article
- Keywords:
Testicular torsion;
Apoptosis;
?-aescin;
bcl-2/Bax
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ?-aescin on the germ cell apoptosis and expression of bcl-2 and Bax proteins in bilateral testes following ipsilateral testicular torsion and detorsion. Methods Thirty adolescent male Wistar rats were randomly divided into control group (C group), model group of testicular torsion and detorsion (M group) and ?-aescin treatment group (T group), each group containing 10 animals. In M and T groups, rats underwent a clockwise 720? ipsilateral testicular torsion for 2 h followed by detorsion, and the rats in T group were intraperitoneally injected with ?-aescin 15 min before detorsion. 48h after operation, bilateral testes were collected, and used to detect cell apoptosis and expressions of bcl-2 and Bax by TUNEL technique and immunohistochemical method, respectively. Results In M group, the apoptotic index of the germ cells and Bax expression of bilateral testes significantly increased compared with C group (P
