A Quantitative and Morphological Study on Cochlear Hair Cell Underwent Diagnostic Ultrasound Exposure in Fetal Guinea Pigs
- VernacularTitle:彩超照射后胎鼠耳蜗毛细胞的定量及形态学观察
- Author:
Xian ZHANG
;
Menghe GUO
;
Aihua YANG
- Publication Type:Journal Article
- Keywords:
Diagnostic ultrasound;
Cochlear hair cell;
SDH;
SEM;
Fetal guinea pig
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of diagnostic ultrasound on cochlear hair cells in fetal guinea pigs. Methods Diagnostic ultrasound was used to irradiate the uteri of early pregnant guinea pigs(the first 15 days of gestion) and late pregnant guinea pigs(the 40th to 45th days of gestion) for 5 minutes daily. The number of cochlear hair cells in fetal guinea pigs was calculated using succinic dehydrogenase (SDH) staining, and computer image analysis system. The morphological change of cochlear hair cells was observed with scanning electron microscope(SEM). Results Compared with non-exposed control group, the number of total hair cells obviously decreased in the two exposed groups (P
