The expression and significance of JNK and p38 MAPK in vascular remodeling of autogenous vein graft
- VernacularTitle:JNK、p38 MAPK在移植静脉血管重塑过程中的表达研究
- Author:
Xinhua HU
;
Jun YANG
;
Chengwei LIU
;
Hongwei ZHANG
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Transplantation;
Veins;
Protein kinases;
Vascular surgical procedures
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of c-Jun N-terminal kinase (JNK) and p38MAPK in autogenous vein graft during vascular remodeling. Methods Autogenous vein graft model was established in 80 Wistar rats. Vein graft samples were harvested at 6th hour, day 1, day 3, day 7, day 14, day 28, day 42 and day 56. Gene expression of JNK and p38MAPK were measured by reverse transcription-PCR and in situ hybridization. Western blot and immunohistochemistry were used to detect the protein expression and phosphorylation of JNK and p38 MAPK. Apoptosis of VSMCs was studied by TUNEL. Results The expression of JNK mRNA and p38 mRNA reached the highest level on the second week (26?10)% and (59?26)% respectively, P
