The effect of EGF on the cell-cycle of estrogen-negative breast cancer cell line MDA-MB-435S
- VernacularTitle:表皮生长因子对ER阴性乳腺癌细胞株细胞周期的影响
- Author:
Hanjin WANG
;
Zhengyan WU
;
Jianmin BIAN
;
Ping FAN
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Epidermal growth factor;
Receptors, estrogen;
NF-kappa B;
Cyclin D1
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which epidermal growth factor (EGF) accelerates the growth of estrogen-negative breast cancer cell line MDA-MB-435S. Methods Under the effect of EGF cyclin D_1 was measured by Western blot and cell-cycle was assayed by FCM. Results EGF enhanced cyclin D_1 expression. Protein kinase C (PKC) inhibitor Go6976 blocked cyclin D_1 over-expression caused by EGF. In EGF group G_0/G_1 was 69.36%, phase S was 22.77% and PI was 0.31. Differences were all significant compared with control (P
