Primary Cell Culture of Human Myometrial Cells In Vitro and Its Application
- VernacularTitle:人子宫平滑肌细胞的原代培养及应用
- Author:
Rongrong ZHANG
;
Zujing YANG
- Publication Type:Journal Article
- Keywords:
Humanmyometrial cell;
Primary cell culture;
Collagenase
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method of primary myometrial cell culture to provide a valuable cell model for a series of research works. Methods Human mometrial cells were isolated by cutting and collagenase digestion, and incubated with DMEM medium and fetal bovine serum. The first passage cells were identified by anti-desmin and-?-action antibodies immunohistochemical staining. Results Primary cultivated human myometrial cells had high purity and viability. Conclusions Primary culture human myometrial cells could obtained by cutting and collagenase digestin.
