Regulation of ERK MAPK in evodiamine-induced A375-S2 cell death
- VernacularTitle:吴茱萸碱诱导A375-S2细胞死亡过程中对ERK激酶的调控
- Author:
Ying ZHANG
;
Qihui ZHANG
;
Lijun WU
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takashi IKEJIMA
- Publication Type:Journal Article
- Keywords:
Evodiamine;
Melanoma;
A375-S cells;
Mitogen-activated protein kinases
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To compare the cytotoxic effect of evodiamine with chemotherapy drugs on A375-S2 cells, and to examine the relationship between the effects of PKC and ERK on evodiamine-induced cell death. METHODS: MTT assay and Western blot analysis were applied. RESULTS: Compared to actinomycin D, cisplatin and 5-FU, evodiamine showed less cytotoxic effects on A375-S2 cells, but it induced more significant inhibition of proliferation in A375-S2 cells incubated with evodiamine for 24 h, followed by continuous culture in drug-free medium. The activation of PKC induced by 10 ?g?L -1 PMA partially blocked evodiamine-induced cell death, which was reversed by PKC and ERK inhibitors. Moreover, evodiamine down-regulated the expressions of ERK and phosphorylated ERK. CONCLUSION: Evodiamine has a strong inhibitory influence on proliferation of A375-S2 cells, even after removal of evodiamine. Evodiamine blocks the protective role of ERK to A375-S2 cells through the downregulation of ERK and phosphorylated ERK expression. [
