Effect of homocysteine on the apoptosis of cultured human umbilical vein endothelial cells
- VernacularTitle:同型半胱氨酸对培养的人脐静脉内皮细胞凋亡的影响
- Author:
Xuewen LIU
;
Xdong LIAO
;
Xiangfeng CONG
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Endothelial cells;
Apoptosis;
Arteriosclerosis
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of homocysteine(Hcy) on the apoptosis of endothelial cells (EC). METHODS: First-passaged human umbilical vein endothelial cells (hUVEC) were cultured with M199 containing 3 mmol/L Hcy. hUVEC apoptosis was detected as follow: demonstration of nuclear changes by Hoechst 33258 staining, agarose gel electrophoresis of DNA fragments, detection of apoptotic cells by flow cytometry following Annexin V-PI doubled stain, Western blot for P53 and Bax protein detection and colorimetry detecting caspase-3 activity. RESULTS: Compared with control, homocysteine induced characteristic apoptotic changes in hUVEC. The chromosomal DNA of hUVEC appeared “DNA ladder” by agarose gel electrophoresis. Apoptotic cells were increased significantly (P
