Effects of hemorrhagic shock on BK_(Ca) channel tyrosine phosphorylation level in mesenteric artery in rats
- VernacularTitle:失血性休克对大鼠血管平滑肌BK_(Ca)通道酪氨酸磷酸化水平的影响
- Author:
Rong ZHOU
;
Liangming LIU
;
Deyao HU
- Publication Type:Journal Article
- Keywords:
Shock, hemorrhagic;
BK_(Ca) channels;
Tyrosine phosphorylation
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of hemorrhagic shock on BK Ca channel tyrosine phosphorylation in rat superior mesenteric artery and the role of nitric oxide (NO) in BK Ca channel tyrosine phosphorylation. METHODS: The hemorrhagic shock model [(35?5) mmHg] was established in rats and the whole lysate of superior mesenteric artery were extracted and analyzed by immune precipition (IP) and immunoblotting. The tyrosine phosphorylation levels of BK Ca channel alpha-subunit in mesenteric artery in hemorrhagic shock rats were investigated, and the modulation of BK Ca channel alpha-subunit tyrosine phosphorylation by NO and its dose-and time-dependended relationships were observed. RESULTS: The tyrosine phosphorylation level of BK Ca channel alpha-subunit in mesenteric artery in rats increased significantly after hemorrhagic shock 2 h and 4 h (P
