Protective effect of ischemic-preconditioning under the mild hypothermia against small intestine ischemia-reperfusion injury in rats
- VernacularTitle:亚低温缺血预处理对大鼠缺血再灌注肠保护作用的研究
- Author:
Shuisheng ZHOU
;
Desheng LUO
;
Hui GAO
;
Jingzhi WAN
;
Ping LEI
- Publication Type:Journal Article
- Keywords:
Intestine, small;
Reperfusion;
Hypothermia;
Ischemic preconditioning;
Gene expression
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the protective effect of ischemic-preconditioning under the mild hypothermia against small intestine ischemia-reperfusion injury in rats and its mechanism. METHODS: Thirty-two rats were randomized into 4 groups (8 rats in each group): sham operated group (Sham), ischemia-reperfusion (I/R) group, ischemic-preconditioning (IP) group, mild hypothermia ischemic-preconditioning (MHIP) group. The wet/dry ratio, Ca 2+ -Mg 2+ -ATPase activity in intestine tissue, the malondialdehyde (MDA) content, activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and total antioxdase (TAX) in blood were determined. Ultrastructure, Bcl-2 and Bax expression in intestinal mucosa tissue were also observed. RESULTS: After I/R, the intestinal tissue wet/dry ratio, the content of MDA, LDH activity, the optic density of Bcl-2 and Bax proteins were significantly higher in I/R group than those in sham group (P
