Methylation of p15INK4B gene and its mechanism in patients with myelodysplastic syndrome and leukemia
- VernacularTitle:骨髓增生异常综合征与白血病细胞p15INK4B基因甲基化及机制研究
- Author:
Hongyan TONG
;
Maofang LIN
- Publication Type:Journal Article
- Keywords:
Genes, p15INK4B;
Myelodysplastic syndromes;
Leukemia;
Methylation;
Methyltransferases
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the relationship between hypermethylation of p15INK4B gene and the pathogenesis of hematopoietic malignances. METHODS: The expression and methylation of p15INK4B gene and the expression of DNA methyltransferase genes (DNMTs) in bone marrow cells from 54 cases with hematopoietic malignances were detected by RT-PCR, Western blot, and methylation-specific PCR. RESULTS: The p15INK4B gene was methylated more often in high-risk myelodysplastic syndrome (MDS) patients, patients at blast phase of chronic myeloid leukemia (CML-BP) and acute leukemia patients than that in low-risk MDS patients (P
