Effects of fosinopril,captopril and valsartan on the expression of tissue factor in human monocytes
- VernacularTitle:福辛普利、卡托普利及缬沙坦对人血单核细胞表达组织因子的影响
- Author:
Hua ZHOU
;
Daifu ZHANG
;
Jiang SHAN
;
Hai SU
;
Huiyan NIU
;
Juan WANG
;
Ping YU
;
Weigang QI
- Publication Type:Journal Article
- Keywords:
Angiotensins;
Thromboplastin;
Monocytes;
Fosinopril;
Captopril
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT 1 antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced. [
