The related gene expression in mesenchymal stem cells differentiating to neuron-like cells
- VernacularTitle:骨髓基质干细胞诱导成神经元样细胞分化相关基因的表达
- Author:
Ying FENG
;
Fukang XIE
;
Liping HU
;
Zhi ZHANG
;
Xiaoqiu ZHOU
- Publication Type:Journal Article
- Keywords:
Stem cells;
Differentiation;
Gene expression;
Neurons;
Mercaptoethanol
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze neuron-related gene expression before and after mesenchymal stem cells (MSCs) differentiating into neuron-like cells. METHODS: MSCs were induced to neuron-like cells with ?-mercaptoethanol. Before inducement and at 8 h after inducement, the total RNA was extracted, then the expression of microtubule-associated protein-2 (MAP-2), growth-associated protein-43 (GAP-43), NSE, nestin and neurofilament (NF) mRNA were detected with RT-PCR. RESULTS: NSE mRNA expressed before and after inducement, MAP-2, GAP-43, nestin and NF mRNA only expressed after inducement. CONCLUSION: The differentiation of MSCs into neuron-like cells may be related to MAP-2, GAP-43, nestin and NF expression. [
