Effects of blocking the expression of PKARⅠ? gene with antisense nucleotide on Shuanglong-Jiegu pill (SLJGP) containing serum-induced osteoblast proliferation
- VernacularTitle:反义封闭PKARⅠ?基因表达对双龙接骨丸含药血清体外促成骨细胞增殖作用的影响
- Author:
Fenyong SUN
;
Qiuhui PAN
;
An HONG
- Publication Type:Journal Article
- Keywords:
Shuanglong-Jiegu pill;
Osteoblasts;
Gene expression;
Nucleotide, antisense
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To further investigate the role of PKARⅠ? in the growth-promoting effects of Shuanglong-Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA-antiPKARⅠ?, a recombinant expressing the antisense sequence of PKARⅠ?, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A. [
