Study on the activity of intestinal mucosal mast cells in rats with dextran sulfate sodium induced coli-tis
- VernacularTitle:肥大细胞在葡聚糖硫酸钠诱导大鼠结肠炎中的致炎作用研究
- Author:
Wenyuan ZHANG
;
Shengxi HAN
;
Hong YANG
- Publication Type:Journal Article
- Keywords:
Intestinal mucosal mast cell;
Histamine;
Tumor Necrosis Factor-a;
Prostaglandin E_2
- From:
Chinese Journal of Digestive Endoscopy
1996;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on the activity of intestinal mucosal mast cells (IMMC) in dextran sulfate sodium ( DSS) induced colitis of rats. Methods Acute colitis was induced by giving 3% DSS orally. Eighteen male rats were randomized into DSS group ( n = 10 ) and normal control group ( n = 8). The rats in DSS control group drank 3% DSS solution from day 1 to day 7; those in normal control group drank water at random. Diarrhea and bloody stool as well as colonic histology were observed. The levels of colonic tissue histamine, tumor necrosis factor-a (TNF-a) and Prostaglandin E2 ( PGE2) determined with fluorimetry and ELISA, and the expression of IMMC in colonic tissue was detected by histochemical stain method. Results Compared with normal control group, the inflammatory symptoms and histologjcal damages of colonic mucosa in DSS group were significantly presented. The level of histamine in colonic tissue was significantly reduced (P
