The Apoptosis Induction Effect of Recombinant Caspase-3 on the Human Osteosarcoma Cell Line SOSP-9901
- VernacularTitle:GFP共表达的重构型Caspase-3基因对人成骨肉瘤细胞的凋亡诱导作用
- Author:
Bing YU
;
Qingyu FAN
;
Lu YAN
- Publication Type:Journal Article
- Keywords:
Recombinant caspase-3;
Apoptosis;
Osteosarcoma cells
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis induction effect of recombinant caspase-3 expression on osteosarcoma cell line SOSP-9901. Methods Recombinant caspase-3 gene was subcloned into the GFP reporter vector pEGFP-C1 to generate the expression vector pEGFP-caspase-3 by DNA recombinant technique. pEGFP-caspase-3 was transfected into human osteosarcoma cell line SOSP-9901 by Lipofectamine 2000. The expression of recombinant caspase-3 was determined by RT-PCR. The morphological changes of transfected cells were observed under flurescent and electronic microscope. The cell survival rate of transfected cells was assayed by MTT method. Results Recombinant caspase-3 gene could be stably expressed in the transfected SSOP-9901 cells. Recombinant caspase-3 could obviously induce SOSP-9901 apoptosis, and inhibit SSOP-9901 cell proliferation in vitro. Conclusion Recombinant caspase-3 could inhibit the growth of the osteosarcoma cell line SOSP-9901 and induce it into apoptosis.
