Application of Anti-contamination in Gene Amplication Technique
- VernacularTitle:基因扩增技术中抗污染的研究
- Author:
Jiande HAN
;
Shaocai DU
;
Feng LIU
- Publication Type:Journal Article
- Keywords:
PCR;
Uracil DNA glycosydase(UDG);
Microplate hybridization;
Anti-contamination
- From:
Journal of Chinese Physician
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of residual UDG on all dU-DNA PCR products. Methods The hybridization percentage of dU-DNA PCR products, which were stored at 37℃ for 20 hours as well as at -20℃,4℃,room temperature and 37℃,respectively, were detected by employing microplate hybridization technique. The effect of inactivating the products at 94 ℃ for 10 minutes on the activity of Taq polymerase was also analysed. Results Compared with the control, A value decreased by 13.281% and 20.557%, respectively after adding 0.2u and 10u UDG (P
