The Role of CpG Sequences in the Induction of Anti-dsDNA Antibodies
- VernacularTitle:CpG序列在诱生抗dsDNA抗体中的作用
- Author:
Zhiming LI
;
Shizheng XU
;
Xiaohua LIN
;
Linlin LIN
;
Yong ZHANG
;
Bingxu LI
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
Antibodies,antinuclear;
Oligon ucleotide array sequence analysis
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CpG sequences on t he induction of anti-dsDNA antibodies and the possible mechanism. Methods Usin g a synthetic oligodeoxynucleotide (CpG-ODN) containing CpG as an adjuvant, nati ve calf thymus DNA (nCTDNA) was used as the mimic self antigen to immunize norma l BALB/c mice. About one week after immunization, anti-dsDNA antibodies were tes ted by ELISA using nCTDNA and native Escherichia coli DNA (nECDNA) as test antig ens. Results The levels of anti-dsDNA antibodies and cytokines including IL-6, IL-12, IFN- but the binding abilities were different significantly. Conclusion The CpG motif can promote the product ion of cross-reactive anti-dsDNA antibodies in normal mice.
