Effects of Extract 5F from Pteris Semipinnata L. on the Apoptosis of Skin Fibroblasts in Patients with Systemic Scleroderma
- VernacularTitle:半边旗提取物5F对系统性硬化病患者成纤维细胞凋亡的影响
- Author:
Zuohuan XIAO
;
Peihua ZHANG
;
Zhihua WU
- Publication Type:Journal Article
- Keywords:
Scleroderma,systemic;
Fibroblast;
Apoptosis;
Pteris semipinn ata
- From:
Chinese Journal of Dermatology
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of extract 5F from Pteris semipinnata L. on the apoptosis of skin fibroblasts in patients with systemic sc leroderma (SSc) and the possible mechanism of its inhibition on the proliferatio n of skin fibroblasts. Methods The cultured SSc skin fibroblasts were used as an experimental model, and the apoptosis of SSc skin fibroblasts before and afte r the extract 5F stimulation was detected by TUNEL assay and fluorescent microsc opy. Results The apoptosis of SSc skin fibroblasts was induced by the extract 5F, and the apoptosis was enhanced along with the increase of the concentration and incubation time of this compound within definite limits. Conclusions These results indicate that the extract 5F can induce the apoptosis of SSc skin fibro blasts, and significantly inhibit the proliferation and collagen synthesis in th ese cells.
