Effect of chronic hypoxia on the gene expression of voltage-gated potassium channels in pulmonary tissue of rats
- VernacularTitle:慢性低氧对大鼠肺组织电压门控钾通道表达的影响
- Author:
Zaiwen FAN
;
Zhenxiang ZHANG
;
Yongjian XU
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Gene expression;
Anoxia;
Lung;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of chronic hypoxia on the expression of voltage-gated potassium channels ? subunits in pulmonary tissue. METHODS: Twelve Wistar rats matched with age and body weight were randomly divided into control and chronic hypoxic groups. The expression of mRNA and protein of Kv1.5, Kv2.1, Kv9.3 in pulmonary tissues were detected with reverse transcription-PCR (RT-PCR) and Western Blot. RESULTS: The expression of Kv1.5, Kv2.1 and Kv9.3 gene were found in pulmonary tissue of rats exposed either to normoxia or chronic hypoxia. Chronic hypoxia decreased mRNA expression of Kv channel subunit (Kv1.5, Kv2.1, Kv9.3) in pulmonary tissue from 0.473?0.022, 0.912?0.084, and 0.319?0.019 to 0.256?0.008, 0.406?0.031 and 0.114?0.009, respectively (P
