Effects of cigarette smoke extract on DNA damage and cell stress in human bronchi smooth muscle cells
- VernacularTitle:香烟提取物对人支气管平滑肌细胞DNA的损伤作用及细胞应激的影响
- Author:
Jungang XIE
;
Yongjian XU
;
Zhenxiang ZHANG
;
Wang NI
;
Shixin CHEN
- Publication Type:Journal Article
- Keywords:
Smoking inhalation injury;
DNA damage;
Heat-shock proteins 70;
Bronchi
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate DNA damage and cell stress (heat shock protein 70 expression) in human bronchi smooth muscle cells by cigarette smoke extract (CSE) in vitro. METHODS: 30 mL smog was dissolved in 1 mL culture medium as stock solution of CSE. Human bronchi smooth muscle cells were cultured 3 hours with 1∶16, 1∶10, 1∶8, 1∶6 and 1∶4 of CSE. The DNA damage and HSP70 expression were determined by single cell gel assay (comet assay) and Western blot, respectively. RESULTS: Associated with rising CSE concentration, DNA damage aggravated. Compared with the untreated group, except 1∶16 of CSE, the level of DNA damage was significantly different (P
