Effect of triptolide on expression of interleukin-13 in lung tissue of rat asthmatic model
- VernacularTitle:雷公藤内酯醇对大鼠哮喘模型肺组织IL-13表达的影响
- Author:
Xiaoyun ZHANG
;
Huanping ZHANG
;
Yongcheng DU
;
Jianying XU
- Publication Type:Journal Article
- Keywords:
Asthma;
Interleukin-13;
Airway inflammation;
Triptolide
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of interleukin-13 (IL-13) in lung tissue of rat asthmatic model and effect of triptolide on expression of IL-13. METHODS: A asthmatic model was established and inflammation changes of lung tissues were examined by haematoxylin-eosin staining. Reverse transcriptio polymerase chain reaction (RT-PCR) was used to semiquantitate the expression of IL-13 mRNA, and enzyme-linked-immuno-sorbent assay (ELISA) was used to detect protein level of IL-13. RESULTS: There was a significant increase in the count of EOS, the expression of IL-13 mRNA and IL-13 protein level in lung tissue of asthmatic rats compared with those of triptolide-treated rats (P0.05). The expression of IL-13 mRNA and IL-13 protein level in triptolide-treated rats were significant lower than that of asthmatic rats (P
