Changes in the expression of Bcl-2 and Fas in HL-60 cells treated with cyclosporine A
- VernacularTitle:环孢菌素A对HL-60细胞Bcl-2和Fas蛋白表达影响的研究
- Author:
Dongjun LIN
;
Renwei HUANG
;
Jun FAN
;
Dongning WANG
;
Guizhen LIN
;
Xudong LI
- Publication Type:Journal Article
- Keywords:
Bcl-2 protein;
Fas protein;
Leukemia;
Apoptosis;
Cyclosporine
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of the expression of Bcl-2 and Fas protein and the apoptosis in HL-60 cells induced by cyclosporine A. METHODS: The expression of Bcl-2 and Fas protein and apoptosis in (HL-60) cells were measured by immunohistochemistry analysis and flow cytometric analysis. RESULTS: There was strong expression of Bcl-2 in HL-60 cells, treatment with cyclosporine A (CsA) for 8-10 h down-regulated the expression of Bcl-2. Fas protein expression in HL-60 cells was very low, CsA induced apoptosis of HL-60 cells, but didn't induce Fas protein expression. CONCLUSION: CsA induces apoptosis in HL-60 cells by down-regulating Bcl-2 expression. [
