Hep-A and Hep-B reduced vascular endothelial growth factor induced breakdown of blood-retinal barrier in mice
- VernacularTitle:环缩酚酞抑制血管内皮细胞生长因子诱导的血视网膜屏障破坏
- Author:
Xiaoling LIANG
;
Haoyu CHEN
;
Yongsheng HUANG
- Publication Type:Journal Article
- Keywords:
Macular edema,cystoid;
Blood-retinal barrier;
E ndothelial growth factor;
Endothelium,vascular;
Phenolphthalein/diagnostic us e
- From:
Chinese Journal of Ocular Fundus Diseases
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Hep-A and He p-B on vascular endothelial growth factor (VEGF)-induced breakdown of blood-r etinal barrier. Methods The mice were subcutaneously injected vehicle, Hep-A or Hep-B 10 mg/kg twice a day for 5 days. Then, 1 ?l of 10 -6 mol/L VEGF were intravitreous injected. After 6 hours, 13.7?10 4 Bq /g 3H-mannital were injected intraperitoneally. The mice were sacrificed and the retinas, lungs, kidneys were removed and examined for radioactivity. The result were analyzed using SPSS software to calculate and compare retina/lung and et ina/kidney leakage ratio among groups of different treatment. Result The retina/lung and retina/kidney leakage ratio were 0.38?0.04 an d 0.21?0.03 respectively in normal mice; increased significantly to 1.05?0.11 and 0.46?0.04 respectively in model mice, both P
