The Biological Activities of Conditioned Medium Derived from Human Dermal Papilla Cells Cultured In Vitro
- VernacularTitle:条件培养基对高传代人毛乳头细胞生长的影响
- Author:
Yang LUO
;
Fei HAO
;
Baiyu ZHONG
;
Yue MAI
;
Xiaoyong JIANG
- Publication Type:Journal Article
- Keywords:
Hair Follicle;
Culture media,conditioned;
Cell culture
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological activities of a conditioned medium for human dermal papilla. Methods Culture medium of the lower passage human dermal papilla cells was collected as the conditioned medium. The growth pattern and the growth curve of the higher passage human dermal papilla cells cultured with conditioned medium were observed in vitro. And the morphology of the co-culture of the higher passage human dermal papilla cells and the lower passage human dermal papilla cells was observed. Results The higher passage human dermal papilla cells, which was cultured with conditioned medium from the lower passage human dermal papilla cells, showed aggregative growth pattern. And the growth curve of the higher passage human dermal papilla cells was much better than that in the control groups (P