The Mechanisms of Tcrolimus on the Migration of Langerhans' Cells
- VernacularTitle:他克莫司影响朗格汉斯细胞迁移机制的研究
- Author:
Ling TANG
;
Yizhi YU
;
Jie CHEN
;
Ming YAN
;
Jun GU
;
Maorong ZHENG
- Publication Type:Journal Article
- Keywords:
Langerhans cells;
Immunosuppressive agents;
Monocyte chemoattractant protein-1;
Cell movements
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of tacrolimus on the migration of Langerhans′ cells. Methods The concentration of MCP-1 in the cultured supernatants of HaCaT cell treated with tacrolimus was determined by ELISA. The expression level of MCP-1 mRNA in HaCaT cell treated with tacrolimus was studied by RT-PCR method. Results The concentration of MCP-1 in HaCaT cell treated with 625 ～ 5 000 ng/mL of tacrolimus was significantly decreased. The expression level of MCP-1 mRNA was also markedly decreased. Conclusions Tacrolimus may probably decrease the expression of MCP-1 in keratinocytes and suppress the chemotactic ability of Langerhans′ cells precursor and Langerhans′ cells, thus inhibiting or diminishing certain local immunoreaction.
