The Impacts of Keratin 17 on the Excretion of IFN- and the Proliferation of T Lymphocytes from Patients with Psoriasis
- VernacularTitle:角蛋白17对银屑病患者T细胞增殖和分泌干扰素?的影响
- Author:
Zhu SHEN
;
Gang WANG
;
Wei LI
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
Psoriasis;
Keratin;
Interferon typeⅡ
- From:
Chinese Journal of Dermatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunological effects of human keratin 17 (K17) on T lymphocytes from psoriatic patients. Methods Total RNA was extracted from the epidermis of psoriatic lesions, and mRNA was reversely transcribed to cDNA. The K17 gene was amplified by PCR and inserted into pGEX-4T-1 expression vector, and then K17 was expressed and purified. T cells were isolated and purified by gra-dient centrifugation and AET-SRBC (2-aminoethylosothiouronium bromide-treated sheep red blood cells) precipitation. MTT assay and direct cell counting method were used to detect the proliferation of T cells. The concentration of IFN- in culture supernatant was measured by ELISA. Results Compared with the control group, K17 significantly enhanced the proliferation of T cells from psoriatic patients and increased the expression level of IFN- (P
