Separation of Cefuroxime Enantiomers by Capillary Zone Electrophoress
- VernacularTitle:头孢呋辛对映体的毛细管区带电泳分离方法研究
- Author:
Rong WANG
;
Zhengping JIA
;
Junjie FAN
;
Jingwen XIE
- Publication Type:Journal Article
- Keywords:
cefuroxime;
enantiomer;
capillary zone electrophoresis
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for separating the cefuroxime enantiomers by capillary zone electrophoresis(CZE).METHODS:Melting capillary column(570mm?75?m)was used with0.04mmol/L hydroxypropyl-?-cyclodextrin and37.5mmol/L NaH 2 PO 4 as buffer solution(pH=6.0).The operation voltage was15kV;temperature was25℃;detecting wavelength was280nm and pressure injection was performed at5kPa for6seconds.RESULTS:The total time for separation and determination was within10min.The recovery was90%～106%.CONCLUSION:This method is simple and rapid,and can be used to determine the content of cefuroxime enantiomers in cefuroxime powder for injection.
