Detection of Aconitine and Mesaconitine and Hypaconitine in blood by LC/MS/MS
- VernacularTitle:血液中乌头碱、次乌头碱、新乌头碱的LC/MS/MS分析
- Author:
Runsheng ZHANG
;
Chen YU
;
Gangyi LIU
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analysis;
Blood;
Aconitine;
Hypaconitine;
Mesaconitine;
LC/MS/MS
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method used for determination of Aconitine and Hypaconitine and Mesaconitine in blood by LC/MS/MS.Methods Extraction of blood sample was conducted by use of 1% thirdchloroactic acid and acetonitrile liquid,and the electrospray fog ionic source,and positive ion MRM scan were employed.Result The linearity correlation coefficients(r)was≥0.992 2.The limit of quantification (LOQ S/N=5) was 2.0 ng/ml for Aconitine,0.5 ng/ml for Hypaconitine,and 0.5 ng/ml for Mesaconitine.The recovery of appended contrast in blank blood sample ranged from 91.25% to 103.1% with coefficient of variation (CV,n=6) less than 10.93%.Conclusion The method of LC/MS/MS is sensitive and reliable,and the sample handling is quick and simple,which was suitable for determination of the Aconitine and Hypaconitine and Mesaconitine in blood sample.
