Determination of Triptolide and Triptonide in plasma by HPLC
- VernacularTitle:高效液相色谱法测定血浆中雷公藤甲素和雷公藤酮
- Author:
Zhaohong WANG
;
Jilin ZHANG
;
Yi HE
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analysis;
Triptolide;
Triptonide;
HPLC;
SPE
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for quantitative determination of triptolide and triptonide in human plasma samples by HPLC.Methods The samples were extracted with Oasis HLB solid phase extraction cartridge and analyzed with reserved phase HPLC coupled with diode array detection.Results Recoveries rates obtained from spiked plasma for the two diterpenoid were higher than 80%.The linearity ranged from 10 ng/ml to 1 000 ng/ml.Detection limits of the method were 3.0ng/ml for triptolide and 4.5ng/ml for triptonide.Conclusion The method is fast and accurate,which is applicable to determination of diterpenoid in cases of suspected poisoning of Triptergium wilfordii Hook.f. in forensic practice.
