Study on the application of differentially methylated X-linked HUMARA in forensic medicine
- VernacularTitle:HUMARA基因座差异甲基化的法医学意义
- Author:
Guisen ZHAO
;
Qingen YANG
;
Daixin HUANG
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
HUMARA locus;
DNA methylation;
methylation-sensitive restriction enzyme(MSRE)
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the Application of X-linked differentially methylated polymorphism site in forensic medicine.Methods X-STR HUMARA was chosen as a model locus.PCR procedures were performed after digestion using methylation-sensitive restriction endonucleases.STR polymorphism of HUMARA was analyzed and compared in samples collected from male and female individuals.Result After digestion with methylation-sensitive restriction enzyme HpaⅡ,no PCR products were obtained from male samples,whereas PCR products from the female samples were normally typed.In monoclonal tumor cell samples from females,only one allele was detected.Conclusion The differentially methylated X-STR HUMARA locus is a novel marker for mixture analysis of mixed stains,sex determination and discrimination of tumor tissues.
