Study on cell DNA damage induced by tetramine using comet assay
- VernacularTitle:毒鼠强诱导细胞DNA损伤的彗星电泳检测
- Author:
Yan LIU
;
Chuanhong ZHU
;
Libin DENG
- Publication Type:Journal Article
- Keywords:
Forensic toxicology;
Tetramine;
DNA damage of cell;
Single cell gel electrophoresis(SCGE);
Comet assay
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect DNA damage in rat lymphocytes and brain cells induced by tetramine and study the toxicological mechanism of tetramine.Methods Lymphocytes and brain cells were separated and collected from healthy rats.DNA damages of cells which were exposed to various doses of tetramine for 60 min was detected using the single cell gel electrophoresis (SCGE) or comet assay.Results These are different degree DNA damages of lymphocytes and brain cells exposed from doses 1/20 LD 50 doses of tetramine to 1/2 LD 50 doses of tetramine.The test groups are very significantly statistical different to the control group(P
