Expression of cyclooxygenase-2 in IL-1?-stimulated mesangial cells is medicated by NF-?B/I?B signal pathway
- VernacularTitle:NF-?B/I?B信号通路在IL-1?诱导的肾系膜细胞环氧化酶-2表达中的作用
- Author:
Guixia DING
;
Aihua ZHANG
;
Songming HUANG
;
Yuanjun WU
;
Li FEI
;
Mei GUO
;
Ronghua CHEN
- Publication Type:Journal Article
- Keywords:
Kidney;
Mesangial cell;
NF-?B;
Prostaglandin-endoperoxide synthase
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of NF-?B/I?B signal pathway in the regulation of (cyclooxygenase-2) (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE_2 concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-?B and degradation of I?B. RESULTS: IL-1? significantly upregulated COX-2 expression and PGE_2 production in HMC. Significant up-regulation of NF-?B activation, nuclear translocation of p65 subunit, and degradation of I?B ? and I?B ? were observed in IL-1?-induced HMC. CONCLUSION: Expression of COX-2 in IL-1?-induced HMC is mediated by NF-?B/I?B signal pathway. [
