Inhibitory effect of antisense eukaryotic expression vectors for c-myc on rat airway smooth muscle cells
- VernacularTitle:反义c-myc真核表达载体的构建及其对大鼠气道平滑肌细胞增殖的抑制作用
- Author:
Yingge LIU
;
Haowen QI
;
Huanzhang LI
;
Mingquan SU
;
Wenbin YU
;
Yueyun MA
- Publication Type:Journal Article
- Keywords:
Genes, myc;
Muscle, smooth;
Airway;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the inhibitory effect of antisense eukaryotic expression vectors for c-myc on rat airway smooth muscle cells. METHODS: Antisense and sense eukaryotic expression vectors for c-myc pcDNA3-myc-antisense and pcDNA3-myc-sense were constructed. Lipofectin was used to introduce antisense and sense eukaryotic expression vectors for c-myc into rat. The inhibitory effect was assayed by MTT cell proliferation assay. Cell cycles were detected by flow cytometry technology. The expression of c-Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense eukaryotic expression vector for c-myc inhibited rat airway smooth muscle cells proliferation. Rat airway smooth muscle cells were prohibited in S phase and the expression of c-Myc was decreased after antisense eukaryotic expression vectors for c-myc were transfected into cells. CONCLUSION: Antisense eukaryotic expression vectors for c-myc inhibit rat airway smooth muscle cell proliferation. [
