Effects of oxidative modification of lipoproteins on blood coagulation and fibrinolysis
- VernacularTitle:脂蛋白的氧化修饰对血凝及纤溶活性的影响
- Author:
Zuyue DENG
;
Bingwen LIU
;
Jing ZHOU
;
Zuhui ZHANG
;
Yu LIU
;
Huai BAI
- Publication Type:Journal Article
- Keywords:
Lipoprotein;
Oxidative modification;
Blood coagulation;
Fibrinolysis
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of oxidative modification lipoproteins on blood coagulation and fibrino (lysis) in vitro. METHODS: Normal human plasma VLDL, LDL and HDL, which were isolated by density gradient ultracentrifugation method, were oxidatively modified by Cu~(2+) and HOCl method. N-VLDL, Ox-VLDL, N-LDL, Ox-LDL, N-HDL, Ox-HDL were added to the reaction system which consisted of mixed fresh normal plasma respectively, prothrombin time (PT), activated partial thrombplastin time (APTT), plasminogen activator inhibitor 1 (PAI-1), tissue plasminogen activator (t-PA) and platelet aggregation were measured according to the direction of the kits. RESULTS: The relative electrophoretic mobility (REM), absorbance at 234nm and TBARS of oxidized VLDL, LDL and HDL mediated by HOCl or Cu~(2+) were significantly higher than that of the control group (P
