DNCB induces colitis and its relation with LMIF activity
- VernacularTitle:DNCB所致小鼠结肠炎与LMIF的关系
- Author:
Ping LIN
;
Lan XIAO
;
Ting ZHOU
;
Jingjing YANG
;
Hui PAN
;
Yusun JIN
;
Encong GONG
;
Lin MEI
- Publication Type:Journal Article
- Keywords:
Colitis, ulcerative;
Leukocyte migration-inhibitory factors;
Dinitrochlorobenzene;
Mice
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to explore the pathogenesis of ulcerative colitis (UC), an experimental colitis in mouse was induced by the hapten dinitrochlorobenzene (DNCB), and the activity of leukocyte migration inhibitory factor (LMIF) was measured at the same time. METHODS: 67 BALB/c mice were randomly divided into control (60% ethanol) and DNCB groups. After they were sensitized by smearing 3.3% DNCB on the abdominal skin, they were challenged with DNCB at concentration of 0.1%, 0.2% and 0.4% respectively by instillation once a day. The weight, stool viscosity and hematochezia were observed and accumulated as disease active index (DAI) score. The pathological changes in colon tissue were judged macropathologically and by means of microscope. LMIF activity was determined by the absorbance (A) of migrated leukocytes. RESULTS: Compared to control group, the increases in DAI accumulate score, pathologic score, and LMIF activity in DNCB groups were observed. CONCLUSION: Mouse colitis was induced by DNCB, which was accompanied by an increase in LMIF activity. [
