Oxidized low density lipoprotein induces the expression of macrophage migration inhibitory factor
- VernacularTitle:氧化型低密度脂蛋白诱导巨噬细胞移动抑制因子的表达
- Author:
Qiuxiong LIN
;
Xiyong YU
;
Zhixin SHAN
;
Shuguang LIN
;
Lan HUIYAO
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Macrophage migration-inhibitory factors;
Arteriosclerosis
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine expression of macrophage migration inhibitroy factor (MIF) gene and protein in macrophages induced by oxidized low density lipoprotein (ox-LDL). METHODS: Macrophages were incubated with ox-LDL at the concentration of 150 mg/L for time course (0-36 h) and with ox-LDL at the different concentrations (0-300 mg/L) for 24 h, expression of MIF mRNA and protein were detected by RT-PCR and ELISA. RESULTS: The results showed that ox-LDL increased MIF gene and protein expression in macrophages in a dose and time-dependent manner. After the exposure of macrophage to ox-LDL, the expression of MIF mRNA level increased consistently with protein. CONCLUSION: MIF may play an important role in atherosclerosis. [
