Effect of epigallocatechin-3-gallate on LPS-induced p38 MAPK pathway in mouse macrophages
- VernacularTitle:没食子儿茶素没食子酸酯对脂多糖激活小鼠巨噬细胞系p38MAPK信号通路的作用研究
- Author:
Lin YAO
;
Xiran ZHANG
;
Shuqin YU
;
Kaihe DU
- Publication Type:Journal Article
- Keywords:
Mitogen-activated protein kinases;
Lipopolysaccharides;
Tumor necrosis factor;
Epigallocatechin gallate
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of epigallocatechin-3-gallate (EGCG) on lipopolysaccharide (LPS)-induced p38 MAPK activation and tumor necrosis factor-? (TNF-?) secretion in macrophages. METHODS: Western blotting was used to detect the phosphorylation of p38 MAPK in mouse macrophages cultured in vitro. Enzyme linked immunosorbent assay was used to determine the secretion of TNF-? in macrophages. Electron microscopy was used to study the effect of EGCG on the structure of LPS. RESULTS: LPS caused activation of p38 MAPK and more production of TNF-?, EGCG inhibited LPS-induced phosphorylation of p38 MAPK and TNF-? production and had no effect on the structure of LPS. CONCLUSIONS: EGCG has no direct effect on LPS, but blocks cellular signal pathway. The inhibition of EGCG on LPS-induced TNF-? production is mediated, at least in part, through blocking of p38 MAPK pathway. [
