Intervention of resveatrol on activated nuclear factor-?B and expression of monocyte chemotactic protein-1 in cultured rabbit aortic smooth muscle cells induced by xanthine and xanthine oxidase
- VernacularTitle:白藜三醇对氧自由基致兔血管平滑肌细胞NF-?B活化及MCP-1表达的影响
- Author:
Hua CUI
;
Zuoyun HE
;
Yuqi GAO
- Publication Type:Journal Article
- Keywords:
NF-kappa B;
Monocyte chemotractant protein-1;
Resveatrol;
Xanthine;
Xanthine oxidase;
Muscle, smooth, vascular
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of resveatrol (RES) among red wine on the proliferation, activity of NF-?B and the expression of monocyte chemotactic protein-1 (MCP-1) induced by xanthine and xanthine oxidase in cultured rabbit aortic smooth muscle cells (SMC). METHODS: SMC proliferation was examined by 3-4, 5-dimethylthiazol-2-yl-2, 5-diphenylte tra zoliumbromide (MTT) metabolism, activity of NF-?B, the protein and mRNA expression of MCP-1 were detected by electrophoretic mobility shift assay (EMSA), immunohistochemitry and in situ hybridyzation in cultured rabbit aortic SMC. RESULTS: 100 ?mol/L-200 ?mol/L resveatrol, an effective composition in red wine, was confirmed to inhibit MTT metabolism and the activity of NF-?B as well as the protein and mRNA expression of MCP-1 in rabbit aortic SMC, which were promoted by the oxygen free radicals induced by xanthine and xanthine oxidase. CONCLUSION: Resveatrol may antagonist oxygen free radicals-induced proliferation and the activity of NF-?B as well as protein and mRNA expression of MCP-1 in cultured rabbit aortic SMC, which might play an important role in preventing atherosclerosis. [
