Effects of angiotensin II and losartan on collagen synthesis in rat hepatic stellate cells
- VernacularTitle:血管紧张素II和洛沙坦对肝星状细胞合成胶原的影响
- Author:
Yijun ZHANG
;
Xishan YANG
;
Pingsheng WU
;
Xiaofeng ZHANG
;
Xiaoqing CHEN
;
Jianying ZENG
- Publication Type:Journal Article
- Keywords:
Angiotensin II;
Losartan;
Liver cirrhosis
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of angiotensin II (Ang II) and AT_(1a) receptor antagonist (losartan) collagen synthesis in rat hepatic stellate cells (HSCs). METHODS: ① Rat HSCs were isolated, cultured and identified. ② Rat HSCs were incubated in the medium with different concentrations of AngII or losartan, then the quantity of collagen was examined by [~3H]-proline release assay. RESULTS: ① The yield of HSCs was 2?10~7-(3?10~7/per) rat, their viability and purity was more than 95% and 90%, respectively. ② The yield of collagen in HSCs significantly got a rise in a concentration-dependent manner when HSCs were incubated with AngII (10~(-6)mol/L-10~(-10) mol/L) (P
