Effect of cyclosporin A on neuropeptide Y-induced proliferation of cultured vascular smooth muscle cells
- VernacularTitle:环孢素A对神经肽Y刺激的大鼠血管平滑肌细胞增殖的影响
- Author:
Desheng LUO
;
Yinghong LI
;
Ke LI
;
Jingping OUYANG
- Publication Type:Journal Article
- Keywords:
Cyclosporin A;
Neuropetide Y;
Vascular smooth muscle cells;
Proliferation;
Calcineurin
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of calcineurin (CaN)-dependent signaling pathway in proliferation of vascular smooth muscle cells (VSMCs) by observing the effect of cyclosporin A (CsA) on proliferation of neuropeptide Y (NPY)-induced rat VSMCs. METHODS: Upon the model of cultured rat VSMCs, the study consisted of three groups: NPYgroup,CsA+NPY group and control group. CaN activity was determinated by enzyme reaction phosphorus measurement. The methods of biochemistry (MTT) and quantitative immunocytochemistry were applied to investigate the proliferation of VSMC and the expression of proliferation cell nuclear antigen (PCNA) in cultured rat VSMCs. (RESULTS:) (Compared) with the control group, the VSMC's CaN activity, proliferation activity and expression of PCNA (by photo densitometry A_(PCNA)) were obviously increased in NPY group (P
