Experimental study of isolation, culture and differentiation of islet stem cells in neonatal rat
- VernacularTitle:新生鼠胰岛干细胞分离、培养和分化的实验研究
- Author:
Rixing BAI
;
Jingqi TIAN
;
Haiyan ZHANG
;
Weihong LI
;
Libo SUN
;
Deheng ZHANG
;
Maomin SONG
- Publication Type:Journal Article
- Keywords:
ISLETS OF LANGERAS;
CELL SEPARATION;
CELLS,CULTURED;
CELL DIFFERENTIATION
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method of isolation, identification and in vitro differentiation of islet stem cells in neonatal rat. Methods The whole pancreata of neonatal rats were digested with collagenase, then,under the (condition) of pH 7.4～7.6,the digested tissue fragments were cultured with serum and serum-free RPMI 1640 (( bFGF), EGF, N2).The whole formation process of new islet cell-like cell masses was examined.The insulin (release) test was used to detect islet function. The expression of nestin was tested by immunocytochemistry. Results The nestin positive cells can be found within 36 h of culture of the digested pancreatic fragments.After addition of bFGF,ECF,N2,nestin positive cells proliferated fast and formed new islets-like cell masses after 18～24 d, and (insulin)ssion could be confirmed. Conclusions The nestin positive cells of pancreatic cells possess the character of islet stem cells, and can form islet like cell masses through culture in vitro.
