The effect of hypothermia on cytokine expression in rats with lipopolysaccharide-induced acute respiratory distress syndrome
- VernacularTitle:中度低温对内毒素性急性呼吸窘迫综合征大鼠肺细胞因子表达的影响
- Author:
Xiao CHEN
;
Yinming ZENG
;
Haozhong DING
- Publication Type:Journal Article
- Keywords:
Hypothermia;
Respiratory distress syndrome, adult;
Endotoxins;
Tumor necrosis factors;
Interleukin-6
- From:
Chinese Journal of Anesthesiology
1995;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hypothermia on cytokine expression in rats with lipopolysaccharide- induced acute respiratory distress syndrome (ARDS), and investigate whether or not hypothermia can inhibit inflammatory reaction and attenuated lung injuries, thereby preventing the development of ARDS. Methods A rat model of ARDS was established by intratracheal instillation of lipopolysaccharide (3 mg/ kg, 0.5 ml, LPS) at 16 h after LPS (1 mg/kg, 0.3 ml) intraperitoneal administration. Thirty-two male Sprague Dawley rats were randomly divided into four groups: ARDS + Normalthermia (AN) , ARDS + Hypothermia (AH) , NS+ Normalthermia (NN), and NS + Hypothermia (NH) groups. At 3 h after ARDS, rats were killed by hemorrhage from carotid artery. Lung lavage was performed, tumor necrosis factor ?(TNF-?) , interleukin-6 (IL-6) concentrations in BALF were measured using enzyme-linked immunosorbent assay (ELISA) .Results Within (2.3?1.6) h the ARDS model in AN group and AH group was successfully replicated. Compared with the NN group, the concentrations of TNF-? and IL-6 increased significantly in the AN group ( P 0.05). Conclusion Hypothermia has inhibitory effect on the expression and release of the pro-inflammatory cytokine (TNF-?, IL-6) in rats with ARDS. Hypothermia may inhibit inflammatory reaction, attenuated lung injuries and serve as a new measure to prevent and treat ARDS.
